MODIFIED LOW-DOSE FORMALIN PRESERVATION: IN THE ANATOMICAL-SURGICAL DISSECTION & PROSECTED ANATOMICAL MODELS: A CASE REPORT
Main Article Content
The development of cadaver preservation has been fast-paced. The subtraction of formalin dose in embalming has been used since many negative factors were found in long-term usage. Preventing putrefaction progress on the cadavers, ensuring no risk of infection when in contact with a dead body, preventing hardening and retention of color tissue and organs, preventing desiccation, inhibit fungal or bacterial growth are the main goals of embalming for anatomical purposes. The new formula has been developed by using low-dose formalin, ethanol, phenol, and sodium chloride (Modified Low-Dose Formalin). This method can be used in practical anatomical-surgical dissection using either cadavers or anatomical models with low-cost maintenance.
Keywords:
Modified Low Dose
Formalin Preservation
Anatomical and clinical dissection
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10. Pustaka Ilmiah Universitas Padjadjaran » Blog Archive » PERBANDINGAN FORMALIN 10% DAN MODIFIKASI LARUTAN LARSSEN DALAM PENGAWETAN JARINGAN TIKUS GALUR WISTAR YANG DITINJAU DALAM STRUKTUR MAKROSKOPIK DAN JUMLAH KOLONI BAKTERI [Internet]. [cited 2024 Dec 24]. Available from: https://pustaka.unpad.ac.id/archives/126877
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17. Sajjacharoenpong K, Chuesiri P. Effectiveness of embalming solutions with partial replacement of formaldehyde and phenol with ethanol and sodium chloride on mice over 18 months. The Thai Journal of Veterinary Medicine [Internet]. 2020 Jun 1 [cited 2024 Dec 24];50(2):233–8. Available from: https://digital.car.chula.ac.th/tjvm/vol50/iss2/14
18. Oruç Zİ, Turan E. Investigation of the use of gelatine-glycerine and gelatine-polyvinyl alcohol mixtures as tissue consistency preservatives in cadavers fixed with modified SEFS solution. Ann Anat [Internet]. 2024 Jun 1 [cited 2024 Dec 24];254. Available from: https://pubmed.ncbi.nlm.nih.gov/38408528/
19. Sam F, Francis DV, Michael JS, Ganesan MP, Rabi S. Replacing formalin with phenoxetol as an immersion medium in a human cadaveric storage facility – A feasibility study. Current Medical Issues. 2024 Jan;22(1):7–13.
20. Watanabe M, Yoneyama Y, Hamada H, Kohno M, Hasegawa O, Takahashi H, et al. The Usefulness of Saturated Salt Solution Embalming Method for Oral Surgical Skills Training: A New Cadaveric Training Model for Bone Harvesting. Anat Sci Educ [Internet]. 2020 Sep 1 [cited 2024 Dec 24];13(5):628–35. Available from: https://pubmed.ncbi.nlm.nih.gov/31608585/
2. Khalil MK. GEL, L V., Giannaris EL, Lee V. Integration of clinical anatomical sciences in medical education: Design, development and implementation strategies. J Clinical Anatomy. 2021 May 3;34(5):785–93.
3. GEAVLETE B, POPESCU R, IORDACHE V, GEORGESCU D, GEAVLETE P. Innovative Cadaver Preservation Techniques: a Systematic Review. Maedica - A Journal of Clinical Medicine. 2023 Mar 15;18(1).
4. Bhanarkar AK, Ray UP. Embalming with Formalin-Benefits and Pitfalls. Scholars International Journal of Anatomy and Physiology Abbreviated Key Title: Sch Int J Anat Physiol. 2022;5(3).
5. Lombardero M, Yllera MM, Costa-e-Silva A, Oliveira MJ, Ferreira PG. Saturated salt solution: a further step to a formaldehyde-free embalming method for veterinary gross anatomy. J Anat [Internet]. 2017 Aug 1 [cited 2024 Dec 24];231(2):309–17. Available from: https://pubmed.ncbi.nlm.nih.gov/28542788/
6. Nam SM, Moon JS, Yoon HY, Chang BJ, Nahm SS. Comparative evaluation of canine cadaver embalming methods for veterinary anatomy education. Anat Sci Int [Internet]. 2020 Sep 1 [cited 2024 Dec 24];95(4):498–507. Available from: https://link.springer.com/article/10.1007/s12565-020-00547-x
7. Arce JE, Contreras E, Vaghani P, Goyal K, Balta JY. The Dissection Experience of Orthopedic Resident: A Comparison between Soft-Preservation and Formalin-Fixation Embalming Techniques. The FASEB Journal [Internet]. 2022 May [cited 2024 Dec 24];36(S1). Available from: https://onlinelibrary.wiley.com/doi/full/10.1096/fasebj.2022.36.S1.R5264
8. Shian SW, Soenggono A, Pramod SV. Comparison of Tissue Preservation using Formalin and Ethanol as Preservative Formula. Althea Medical Journal [Internet]. 2016 Sep 30 [cited 2024 Dec 24];3(3):359–63. Available from: https://journal.fk.unpad.ac.id/index.php/amj/article/view/880
9. Mrożek A, Sędzik M, Rubin J, Buras S, Tarkowski V, Domagała Z. Cadaver preserving methods – is it possible to do anything better? Medical Journal of Cell Biology. 2022 Dec 1;10(4):124–8.
10. Pustaka Ilmiah Universitas Padjadjaran » Blog Archive » PERBANDINGAN FORMALIN 10% DAN MODIFIKASI LARUTAN LARSSEN DALAM PENGAWETAN JARINGAN TIKUS GALUR WISTAR YANG DITINJAU DALAM STRUKTUR MAKROSKOPIK DAN JUMLAH KOLONI BAKTERI [Internet]. [cited 2024 Dec 24]. Available from: https://pustaka.unpad.ac.id/archives/126877
11. Gosomji IJ, Omirinde JO, Hena SA, Wanmi N, Azeez IA. Saturated Salt Solution an Alternative Reagent in Reducing Formaldehyde Concentration in Embalming. 2018 Jan 5 [cited 2024 Dec 24]; Available from: http://irepos.unijos.edu.ng/jspui/handle/123456789/2391
12. Shah A, Jhawar S, Goel A, Goel A. Corpus Callosum and Its Connections: A Fiber Dissection Study. World Neurosurg [Internet]. 2021 Jul 1 [cited 2024 Dec 24];151:e1024–35. Available from: https://pubmed.ncbi.nlm.nih.gov/34033953/
13. Soonklang N, Saowakon N. Evaluation of formaldehyde exposure among gross dissection after modified embalming solution and health assessment. Environmental Science and Pollution Research [Internet]. 2022 Sep 1 [cited 2024 Dec 24];29(43):65642–54. Available from: https://link.springer.com/article/10.1007/s11356-022-19704-y
14. Tiruneh C. Acute adverse effects of formaldehyde treated cadaver on new innovative medical students and anatomy staff members in the dissection hall at Wollo University, Northeast Ethiopia. Adv Med Educ Pract [Internet]. 2021 [cited 2024 Dec 24];12:41–7. Available from: https://www.tandfonline.com/action/journalInformation?journalCode=dame20
15. Lang I, Bruckner T, Triebig G. Formaldehyde and chemosensory irritation in humans: a controlled human exposure study. Regul Toxicol Pharmacol [Internet]. 2008 Feb [cited 2024 Dec 24];50(1):23–36. Available from: https://pubmed.ncbi.nlm.nih.gov/17942205/
16. Kang DS, Kim HS, Jung JH, Lee CM, Ahn YS, Seo YR. Formaldehyde exposure and leukemia risk: a comprehensive review and network-based toxicogenomic approach. Genes Environ [Internet]. 2021 Dec 1 [cited 2024 Dec 24];43(1). Available from: https://pubmed.ncbi.nlm.nih.gov/33845901/
17. Sajjacharoenpong K, Chuesiri P. Effectiveness of embalming solutions with partial replacement of formaldehyde and phenol with ethanol and sodium chloride on mice over 18 months. The Thai Journal of Veterinary Medicine [Internet]. 2020 Jun 1 [cited 2024 Dec 24];50(2):233–8. Available from: https://digital.car.chula.ac.th/tjvm/vol50/iss2/14
18. Oruç Zİ, Turan E. Investigation of the use of gelatine-glycerine and gelatine-polyvinyl alcohol mixtures as tissue consistency preservatives in cadavers fixed with modified SEFS solution. Ann Anat [Internet]. 2024 Jun 1 [cited 2024 Dec 24];254. Available from: https://pubmed.ncbi.nlm.nih.gov/38408528/
19. Sam F, Francis DV, Michael JS, Ganesan MP, Rabi S. Replacing formalin with phenoxetol as an immersion medium in a human cadaveric storage facility – A feasibility study. Current Medical Issues. 2024 Jan;22(1):7–13.
20. Watanabe M, Yoneyama Y, Hamada H, Kohno M, Hasegawa O, Takahashi H, et al. The Usefulness of Saturated Salt Solution Embalming Method for Oral Surgical Skills Training: A New Cadaveric Training Model for Bone Harvesting. Anat Sci Educ [Internet]. 2020 Sep 1 [cited 2024 Dec 24];13(5):628–35. Available from: https://pubmed.ncbi.nlm.nih.gov/31608585/
